SOLVED: You will need to determine your insert amount based on the table using the NEB ligation calculator. If you do not have enough insert DNA based on the calculations, Stanley cut
New England Biolabs (UK) Ltd - Quick Ligation™ Kit
Ligation time courses for defined substrates. Ligation of threebase... | Download Scientific Diagram
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NEB Interactive Tools - New England Biolabs GmbH
NEBioCalculator® - Using the Ligation module | NEB
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When Fidelity Matters: A frank discussion about ligase fidelity
Ligation Calculator
